How can you determine the affect of temperature on the metabolism of yeast? Brainstorm ideas among the members of your group.
Decide on one hypothesis that you wil test. Your hypothesis might be that low temperature slows down the metabolic activity of yeast, or that a high temperature speeds up the metabolic activity
In this BioLab, you will:
- Measure the rate of yeast metabolism using a BTB color change as a rate indicator.
- Compare the rates of yeast metabolism at several temperatures.
- Use the Internet to collect and compare data from other students.
- Bromothymol blue solution (BTB)
- Small test tubes (4)
- Large test tubes (3)
- One-hole stoppers with glass tube inserts for large test tubes (3)
- Yeast/white corn syrup mixture
- Water/white corn syrup mixture
- Test-tube rack
- 250 mL beakers (3)
- Ice cubes
- Celsius thermometer
- Hot plate
- 50 mL graduated cylinder
- Glass-marking pencil
- 10 cm rubber tubing (3)
- Aluminum foil
Always wear goggles in the lab. Be careful in attaching rubber tubing to the glass tube inserts in the stoppers. Avoid touching the top of the hot plate. Wash your hands thoroughly after cleaning
out test tubes at the end of your experiements.
Use the Skill Handbook if you need additional help with this lab.