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Internet Biolab
Click the Techie Toolbox for tips and help to make the most of your time on the Web.
Unit 8: Invertebrates
Chapter 25: What Is an Animal?  
Zebra Fish Development
The zebra fish (Danio rerio) is a common freshwater fish sold in pet shops. They are ideal animals for study because they undergo developmental changes quickly and major stages can be observed within hours after fertilization.
Preparation
Problem
What do the developmental stages of the zebra fish look like?

Objectives
In this BioLab, you will:

  • Observe stages of zebra fish development.
  • Record all observations in a data table.
  • Use the Internet to collect and compare data from other students.
Materials
  • Zebra fishes (males and females)
  • Prepared Aquarium
  • Binocular microscope
  • Petri dish
  • Dropper
  • Wax pencil
  • bulb baster
  • beaker
Data Sources

Safety Precautions
CAUTION Always wear safety goggles in the lab. Use caution when working with a binocular microscope and glassware.

Skill Handbook
If you need help with this lab, refer to the Skill Handbook.


Procedure
1. Print the data table.

2. Use the bulb baster to transfer water and fish embryos from the aquarium to a beaker. Allow the embryos to settle to the bottom.

3. Use a wax pencil to write your name and class period on the edge of the lid of your petri dish. Use the dropper to half fill the bottom of your petri dish with aquarium water, and then to transfer several embryos from the beaker to your petri dish. Place the lid on your petri dish.

4. Your teacher will tell you the approximate time that fertilization occurred. Record the age of the embryos in your data table as hpf (hours past fertilization).

5. Observe the embryos under the microscope. In your data table, diagram what you observe.

6. Post your data.

7. Continue to observe your embryos daily for one week. Note when new organs appear and when movement is first seen. If you want to continue observing developmental changes, ask your teacher for instructions.
CAUTION: Wash your hands with soap and water immediately after each observation.

8. Clean all equipment as instructed by your teacher, and return everything to its proper place. Dispose of the water and embryos properly. Wash your hands thoroughly.

Post Your Data

Date hpf Observations

*City


*State


*School


*Required
field

Analyze and Conclude
1. Explain why zebra fish are ideal animals for studying embryonic development.

2. Thinking Critically Explain why you may not have been able to see stages such as a blastula or gastrula.

3. Collect and Organize Data Visit
for links to internet sites that will help you complete sequences of the major changes during development of zebra fishes:

a. between 1 and 10 hpf. Include labeled diagrams of these changes.
b. between 10 and 28 hpf. Include labeled diagrams.
c. between 28 and 72 hpf. Include labeled diagrams.

4. Error Analysis Suggest how you could change the experiment’s design to allow for observing blastula and gastrula stages.